.sql database file protection from user outside of the application access

Using SBS 2011 on a PE T410, is it possible for admin to allow read write access to a .sql database file, by way of an application, but not allow the file to be opened, copied or deleted by a user of the application (out side of the application)?

Pro Support says this is engineer stuff! because the server is out of dated support! 

The vertical package application requires read - write access to the .sql database file, to use data in the application.

I think it is necessary to prevent an user from being able to copy or delete the .sql database file in the windows directory!
It is OK for user to use the application to read and write to the database, but not given any rights to access in the windows directory.

I hope my question makes sense!

Thanks for your help!!

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