Our autosize settings are set to keep a minimum of 110 live machines with a demand buffer of 32.
When users log off the machine gets deleted and removed from the broker and hyper-v. However, it isn't then re-created. If i enable maintenance mode and drain the cloud, i can then disable maintenance mode and it builds all the desktops as it should.
Once users log off and the machines are not re-created we are then running out of machines and users are unable to log on.